Generally the presence of plankton in the waters will be affected by the type of waters (flowing or stagnant), chemical quality Dahn physics waters (eg temperature, brightness, flow, pH, CO2 content is free, etc.) and their competitors plankton predators.
In the stagnant waters where plankton will vary from time to time and different in occupied space or body of water, while the water flowing elements of time and space are relative no real role. This causes the sample to observations of aquatic biological parameters vary.
Water sampling for observation plankton parameter consists of several methods:
a. filtration (filtration method) using a plankton net with mesh sizes adjusted to the desired plankton classification. the amount of water being stored varies 5-50 l depending on the density of plankton that can be seen from the color of the water. Samples were taken using a sampling tool with a certain volume, then filtered using planktonnet. plankton samples deposited in the filter was transferred into the collection bottles and then preserved using formalin or alcohol prior to the identification of plankton under a microscope.
b. settling water sample (sedimentation method) using the tube container
c. The centrifuge method is less desirable because it is not portable
Observations plankton as a biological parameters generally include plankton diversity and abundance of plankton contained in a body of water. calculation of the abundance of plankton can use:
(1) Haemocytometer, observations with this instrument is intended for phytoplankton or microscopic plankton, on a microscope with a magnification of 100 x. Usually used for computation (counting) Phytoplankton with size <10 lm.
Saedgwick rafter plankton observation cell is a device most commonly used for identification of plankton, because it has a relatively larger capacity, so it can be used for identification of phytoplankton and zooplankton are microscopic. Volume Sedgwick rafter proper cell 1 (one) cc or 1 cm3, comprising a length of 50 mm, a width of 20 mm and a thickness of 1 mm.