a) Examination of the situation by way of organoleptic color, smell, taste and texture.
b) Determination of water content with the oven method, the principle of weight loss on heating to 105oC regarded as the water content contained in the sample. Distillation method with the principle of separation of water with organic solvents azeotrapik
c) Determination of ash content with the principles of the ashing process organic matter is decomposed into water and CO2, but no organic material.
d) Determination of food coloring with polyamide column method with dye absorption principle example by dissolving polyamide with dye that has been free of the impurities in methanolic NaOH. In particular pH and after concentrates, benchmarking dye with the dye sample standard paper chromatography performed.
e) Determination of artificial sweetener (saccharin) with Test Resolsinol, the principle will Saccharin gives green fluorescence when reacted with NaOH resolsinol and excessive.
f) The determination of artificial sweeteners (cyclamate) with precipitation, with the principle of formation of a white precipitate from the reaction between BaCl2 Na2SO4 (derived from the reaction between cyclamate with NaNO2 in an atmosphere of strong acid) showed cyclamate.
g) Metal Contamination of Cadmium (Cd) and lead (Pb), namely destruction example by dry ashing at a temperature of 450 ° C followed by dissolution in acid solution. Dissolved metal calculated using the tool Atomic Absorption Spectrophotometer (AAS) with a maximum wavelength of 228.8 nm to 283.3 nm for Cd and Pb.
h) metal contaminants Mercury (Hg) is a reaction between mercury compounds with NaBH4 or SnCl2 under acidic conditions to form the atomic gas Hg. Total Hg formed Hg proportional to the absorbance is read using Atomic Absorption Spectrophotometer (AAS) without flame at a wavelength maximum of 253.7 nm.
i) microbial contamination: Figures plates that total aerobic bacterial growth mesophyll after sample was incubated in the appropriate seeding for 48 hours at a temperature (35 ± 1) ° C.
j) microbial contamination: Escherichia coli is Escherichia coli growth is characterized by the formation of gas in the Durham tube, followed by biochemical tests and further referenced in Table APM (Score Most Likely).
Sampling method in accordance with SNI 19-0428-1989, sampling solids.
Equipment sample preparation
– Mortar porcelain;
How solid sample preparation
Take for example the diagonal system, collected to obtain a homogeneous sample. Make into a rectangular shape, then divide into two diagonally into four parts. Take two parts facing each other, and then for four more and the next is to do like the workmanship above in order to obtain sufficient quantities for analysis. If the shape of the example is not smooth, grind the sample until smooth.