Determination of ammonia-nitrogen used indophenol method (method phenate). This method of giving out a good result for the waters of the pool water. reagent used is phenate (phenol), chlorox (oxidizing solution) and manganese sulphate. phenol and hypochlorit (chlorox) action in the solution is alkaline-forming phenylquinone monoimine which would then be reacted with ammonia to indophenol blue. blue color density comparable to existing levels of ammonia.
Among the various methods used in determining the ammonia, the most simple is the way of direct Nessler. This method is commonly used on samples that are expected to have a high content of ammonia. A more thorough involves distillation of ammonia and the use of a spectrophotometer.
Determination of ammonia with Nessler reagent. Determination of ammonia relying on the fact that the ammonia ion (NH4 +) gives a yellowish brown color with Nessler reagent, and that the color intensity berbading directly with the amount of ammonia that is.
(1) Reagent
All reagents are made using anhydrous ammonia
(2) Reagent Nessler
Dissolve 10 grams of mercury iodide anhydrous (HgI2) and 7 grams of anhydrous potassium iodide in a small amount of water. Add this mixture with stirring regularly into a cold solution of 16 grams of NaOH in 50 ml of anhydrous ammonia. Dilute to 100 ml. Store in a bottle gelap.Apabilaraksayodida not adadapat also dibuar Nessler reagent using mercury chloride as below.
Dissolve 50 grams of KI in 35 ml of anhydrous ammonia. Add a saturated solution of mercury chloride until there is little sediment. Make a solution of KOH 9 N. Let clear by deposition, Add 400 ml of 9 N KOH solution is clear mercury iodide into campuranlarutan kaliun chloride. Dilute to 1 liter. Allow to clear. Store in a dark bottle and left in the dark.
(3) Ammonium Chloride Parent
Dissolve 3.818 grams of anhydrous ammonium chloride in 1 liter of anhydrous ammonia. 1 ml of this solution containing 100 mg of nitrogen ammonia
(4) Ammonium Chloride Standard
Dilute 10 ml solution of ammonium chloride stem into 1 liter. 1 ml of this solution equal to 10 mg or 12.2 mg ammonium nitrogen ammonia.
(5) 50% Sodium Potassium Lartan tartaric.
Dissolve 50 grams of potassium sodium tartaric in 100 ml of warm anhydrous ammonia.
(6) Solution Lead Acetate 10%
Larutka 10 grams of lead acetate in 100 ml of water.
Ways of working :
(1) Take 1 ml of ammonium chloride in a Nessler tube. Nessler tubes made specifically for color measurement optic. When there is no tube Nessler tube testers use anything made of clear glass. All tubes were used for a comparison of color must be the same size and quality.
(2) Dilute the standard solution to 100 ml. Add 2 ml Nessler reagent.
(3) If a 100 ml sample colorless same in another tube, and add 2 ml Nessler reagent thereto.
(4) Where the water sample became cloudy on the addition of Nessler reagent, stop.
(5) To the fresh sample add 2 ml of a mixture of sodium and potassium, tartaric 50% Nessler reagent with the same volume.
(6) Let both the standard and the sample for 10 minutes. The yellow color that occurs directly compare with the number of existing ammonia.
Estimated amount of ammonia can be made based on the intensity of the color. Sorrel characterize the presence of more than 5 mg / liter of ammonia. Striking yellow color characterizes the ammonia concentration between 1 to 5 mg / liter. The yellow color is almost unobservable mancirikan ammonia is less than 0.1 mg / liter.