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Sampling Technique

Some things to consider in taking the sample are:
1) Fixation
Fixation is the process of sample preservation using preservatives that material taken can be processed by PCR. The principle of choosing the type of preservative:
a) easy in the handling, storage and transport,
b) Not reduce the sensitivity of diagnosis,
c) Easily available and relatively inexpensive.

There are two commonly used preservation method that is
a) fixation in alcohol solution,
b) Freezing in temperatures below 20oC.
Each technique has its advantages and disadvantages. However, it is suggested that in the pickling process using a solution of 70% alcohol by volume ratio of the sample compared to the preservative 1: 10.

2) Sampling Equipment
Sampling equipment in question is the equipment directly used in sampling that sample bottles and surgical instruments. Any equipment to be used must be disinfected beforehand. Surgical instrument disinfection process starts from:
a) Cleaning equipment with tissue paper
b) Rinsed with distilled water
c) disinfection with 70% alcohol
d) Heating with a Bunsen flame

3) How Sampling
Sampling right must pay attention to the type and number of samples. Samples can be taken from seed, mature fish, and parent. Samples of the seeds in the form of the body as a whole, while the adult fish gills and stem can be. Minimum number of samples taken depends on the level pravelensi infected fish viral diseases, however, to diagnose cases of the viral disease premises PCR technique is based on empirical experience.
Total sample of the population with more than 100,000 birds, is;
a) For seed 150 animals
b) For adult fish as much as 5 individuals,
c) To the parent of sampling individuals (taken slices gills without turning off the mains).

3) How Sampling
Sampling right must pay attention to the type and number of samples. Samples can be taken from seed, mature fish, and parent. Samples of the seeds in the form of the body as a whole, while the adult fish gills and stem can be. Minimum number of samples taken depends on the level pravelensi infected fish viral diseases, however, to diagnose cases of the viral disease premises PCR technique is based on empirical experience.
Total sample of the population with more than 100,000 birds, is;
a) For seed 150 animals
b) For adult fish as much as 5 individuals,
c) To the parent of sampling individuals (taken slices gills without turning off the mains).

Sample Delivery Techniques
Fish samples have been collected and diawet with alcohol 70% – 90%, it should be immediately sent to the nearest laboratory capable of performing a diagnosis of viral diseases by PCR. Samples should be packed as possible so it does not leak during shipment. The emergence of disease in fish is the result of a complex interaction between the three components of the aquatic ecosystem is weak fish, fierce pathogens, and environmental quality was bad. Therefore, fish health management strategies should focus on revamping integrated manner.

Mechanical control
Provision of seeds to areas free of viral diseases should be taken from areas free of viral diseases. While the provision of seeds to areas that have been infected with the virus taken the seeds produced from the parent who survived the outbreak because the seeds produced from the parent it is considered to have immunity to viral diseases.

eradication of Pathogens
Virus activity is the destruction of the carrier (water and careers). Areas that have been infected with KHV disease eradication must be done especially in enclosed cultivation systems.